Doaaasker

 

Adminstrator

 

للاسف انا مش بيو

احمد مصيلحي ممكن يكون عارف !!

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white ice

 

طيب بصى أنا مش متأكده الإجابه دى صح تماما ولا لأ

بس شوفيها كده وقوليلى عقبال ما أى حد تانى عنده إجابه يدخل ويحلهولك

• Biuret - This method is the most linear because its color depends on a direct complex between the peptide bonds of the protein and Cu(2+) ion. It is not highly sensitive since the complex does not have a high extinction coefficient. You will use this method to learn making a standard curve - your Biuret Standard Curve should be nearly perfectly linear!
• Folin (Lowry) - The Folin assay (also called Lowry method after the scientist who discovered it and published a description of the method in 1951) is dependent on the presence of aromatic amino acids in the protein. First, a cupric/peptide bond complex is formed and then this is enhanced by a phosphomolyboda te complex with the aromatic amino acids. Overall, about 10 to 50 times more sensitive than the Biuret method. The Folin Standard Curve is usually not perfectly linear, but in your expt in this class your results are usually quite linear if the assay is properly done. Many substances interfere with the Folin assay for protein.
• Bradford assay is based on a blue dye (Coomassie Brilliant Blue) that binds to free amino groups in the side chains of amino acids, especially Lys. This assay is as sensitive as the Folin assay and can be done so that it is more sensitive, especially using the commercial kits available from Bio-Rad and some other chemical companies. This method can yield quite linear standard curves with BSA but often is found to be not quite linear. Few substances interfere with this assay. The assay can also be done in the presence of detergent which makes it useful for determining protein concentrations for membrane proteins.

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Doaaasker

 

ميرسى جداااااااااااا يادؤدؤ ملناش غيرك هنا ف المنتدى قشطات ياجميل بس اخر نقطة دى حاجة تانية غير الفولين والبيروت صح؟

white ice

 

ربنا يخليكى يا دودو

بس والله آخر نقطه دى مش متأكده والله
علشان ماقولكيش حاجة وتطلع غلط

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Doaaasker

 

طب معلش يادودو غلاسة بس فسريهالى كدا بس عربى ولا اكلمك احسن؟عشان بس الحل متتداخل مع حل واحدة صحبتى